Generating Single Cell–Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9-Reference-Cited by-同舟云学术

Generating Single Cell–Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9

Published:2019-07-26 Issue:1 Volume:128 Page:
ISSN:1934-3639
Container-title:Current Protocols in Molecular Biology
language:en
Short-container-title:Current Protocols in Molecular Biology

Author:

Giuliano Christopher J.¹²³,Lin Ann¹²,Girish Vishruth¹²,Sheltzer Jason M.¹

Affiliation:

1. Cold Spring Harbor Laboratory Cold Spring Harbor New York

2. Stony Brook University Stony Brook New York

3. Massachusetts Institute of Technology Cambridge Massachusetts

Funder

National Science Foundation

Cold Spring Harbor Laboratory

Publisher

Wiley

Subject

General Medicine

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/cpmb.100

Reference55 articles.

1. Abcam. (2018a accessed December 26).Knockout (KO) validation results. Retrieved fromhttps://www.abcam.com/primary‐antibodies/knockout‐ko‐validation‐results.

2. Abcam. (2018b accessed December 31.)Western blot protocol. Retrieved fromhttps://www.abcam.com/protocols/general‐western‐blot‐protocol.

3. Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3

4. CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes

5. The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity

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