Generating Single Cell–Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9
Author:
Affiliation:
1. Cold Spring Harbor Laboratory Cold Spring Harbor New York
2. Stony Brook University Stony Brook New York
3. Massachusetts Institute of Technology Cambridge Massachusetts
Funder
National Science Foundation
Cold Spring Harbor Laboratory
Publisher
Wiley
Subject
General Medicine
Link
https://onlinelibrary.wiley.com/doi/pdf/10.1002/cpmb.100
Reference55 articles.
1. Abcam. (2018a accessed December 26).Knockout (KO) validation results. Retrieved fromhttps://www.abcam.com/primary‐antibodies/knockout‐ko‐validation‐results.
2. Abcam. (2018b accessed December 31.)Western blot protocol. Retrieved fromhttps://www.abcam.com/protocols/general‐western‐blot‐protocol.
3. Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3
4. CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes
5. The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity
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