Affiliation:
1. Department of Dermatology University of Massachusetts Chan Medical School Worcester Massachusetts
Abstract
AbstractTraditional skin sampling methods include punch or shave biopsies to produce a solid tissue sample for analysis. These biopsy procedures are painful, require anesthesia, and leave permanent scars. This unit describes a suction blister skin biopsy method that can be used in place of traditional biopsy methodologies as a minimally invasive, non‐scarring skin sampling technique. The induction of suction blisters uses an instrument with a chamber that applies negative pressure and gentle heat to the skin. Blister formation occurs within 1 hr, producing up to five blisters, each 10 mm in diameter per biopsy site. Blister fluid can be extracted and centrifuged to retrieve cells from the epidermis and upper dermis for flow cytometry, single‐cell RNA sequencing, cell culture, and more without the need for digestion protocols. In addition, the blister fluid can be used to measure soluble proteins and metabolites. This unit describes the preparation of supplies and subjects, the suction blister biopsy procedure and blister formation, fluid extraction, and post‐blistering care. © 2024 Wiley Periodicals LLC.Basic Protocol 1: Preparation of supplies and subjectBasic Protocol 2: Suction blister biopsy procedure and formationBasic Protocol 3: Blister fluid extractionBasic Protocol 4: Post‐blister care and clean up