Affiliation:
1. Henan Sesame Research Center, Henan Academy of Agricultural Sciences Postgraduate T&R Base of Zhengzhou University Zhengzhou People's Republic of China
2. School of Agricultural Sciences Zhengzhou University Zhengzhou People's Republic of China
3. Henan Key Laboratory of Specific Oilseed Crops Genomics (Henan Sesame Research Center, Henan Academy of Agricultural Sciences) Zhengzhou People's Republic of China
4. The Shennong Laboratory Zhengzhou People's Republic of China
Abstract
AbstractSesame (Sesamum indicum L.), 2n = 2x = 26, is an important oilseed crop with high heterosis potential. In this study, we explored the histological and genetic characters and causal gene of a male sterile sesame mutant, ms1812 (ms3). The microspore abortion of ms1812 that led to male sterility (MS) occurred at the tetrad stage of microspore development. No normal pollens were formed and observed in anther chambers of ms1812 at the microspore mature stage. With the aid of high‐efficient association mapping and the sesame var. Yuzhi 11 reference genome, we identified the single nucleotide polymorphism 10505640 on SiChr.3 segregating with the MS and the mutated gene, Si4cll1, controlling the recessive MS in sesame. Si4CLL1 encodes a 4‐coumarate‐CoA ligase‐like protein. The 1292th nucleotide (T1292) of Si4CLL1 mutated into A1292, resulting in the amino acid change from Ile239 to Lys239. Si4CLL1 is mainly expressed in young developing buds. Compared with the wild type, the transcript abundance of Si4cll1 in the developing buds of ms1812 reduced significantly, which might affect the phenylpropanoid biosynthesis pathway and finally, the lignin formation of microspore cell wall. These findings provide key new information for deciphering the molecular mechanism underlying MS and hybrid variety breeding assisted by MS in sesame.
Subject
Agronomy and Crop Science