Affiliation:
1. School of Instrument Science and Opto‐Electronics Engineering of Beijing Information Science & Technology University Beijing China
2. Institute of Medical Technology Peking University Health Science Center Beijing China
3. Med‐X Research Institute and School of Biomedical Engineering Shanghai Jiao Tong University Shanghai China
4. International Cancer Institute Peking University Beijing China
Abstract
AbstractFlow cytometry (FC) is a versatile tool with excellent capabilities to detect and measure multiple characteristics of a population of cells or particles. Notable advancements in in vivo photoacoustic FC, coherent Raman FC, microfluidic FC, and so on, have been achieved in the last two decades, which endows FC with new functions and expands its applications in basic research and clinical practice. Advanced FC broadens the tools available to researchers to conduct research involving cancer detection, microbiology (COVID‐19, HIV, bacteria, etc.), and nucleic acid analysis. This review presents an overall picture of advanced flow cytometers and provides not only a clear understanding of their mechanisms but also new insights into their practical applications. We identify the latest trends in this area and aim to raise awareness of advanced techniques of FC. We hope this review expands the applications of FC and accelerates its clinical translation.
Funder
National Natural Science Foundation of China
Subject
General Physics and Astronomy,General Engineering,General Biochemistry, Genetics and Molecular Biology,General Materials Science,General Chemistry