A Small‐Molecule Fluorescence Probe for Nuclear ATP

Author:

Tamima Umme1ORCID,Sarkar Sourav1,Islam Md Reyazul2ORCID,Shil Anushree1,Kim Kyeong Hwan1,Reo Ye Jin1,Jun Yong Woong1,Banna Hasanul2,Lee Soobin1,Ahn Kyo Han1ORCID

Affiliation:

1. Department of Chemistry Pohang University of Science and Technology 77 Cheongam-Ro, Nam-Gu Pohang Gyungbuk 37673 (Republic of Korea

2. Division of Integrative Biosciences and Biotechnology Pohang University of Science and Technology 77 Cheongam-Ro, Nam-Gu Pohang Gyungbuk 37673 (Republic of Korea

Abstract

AbstractFluorescence monitoring of ATP in different organelles is now feasible with a few biosensors developed, which, however, show low sensitivity, limited biocompatibility, and accessibility. Small‐molecule ATP probes that alleviate those limitations thus have received much attention recently, leading to a few ATP probes that target several organelles except for the nucleus. We disclose the first small‐molecule probe that selectively detects nuclear ATP through reversible binding, with 25‐fold fluorescence enhancement at pH 7.4 and excellent selectivity against various biologically relevant species. Using the probe, we observed 2.1–3.3‐fold and 3.9–7.8‐fold higher nuclear ATP levels in cancerous cell lines and tumor tissues compared with normal cell lines and tissues, respectively, which are explained by the higher nuclear ATP level in the mitosis phase. The probe has great potential for studying nuclear ATP‐associated biology.

Funder

Ministry of Education

Publisher

Wiley

Subject

General Medicine

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