A new mixed-mode model for interpreting and predicting protein elution during isoelectric chromatofocusing

Author:

Choy Derek Y.C.1,Creagh A. Louise1,von Lieres Eric12,Haynes Charles1

Affiliation:

1. Michael Smith Laboratories; and the Department of Chemical and Biological Engineering; University of British Columbia; 231 Michael Smith Building Vancouver BC Canada V6T 1Z4

2. Institute of Bio- and Geosciences IBG-1: Biotechnology; Jülich GmbH; Jülich Germany

Funder

Natural Sciences and Engineering Research Council of Canada

Publisher

Wiley

Subject

Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

Reference28 articles.

1. Quasi-linear pH gradients for chromatofocusing using simple buffer mixtures: Local equilibrium theory and experimental verification;Bates;J Chromatogr A,1998

2. High-performance chromatofocusing using linear and concave pH gradients formed with simple buffer mixtures I. Effect of buffer composition on the gradient shape;Bates;J Chromatogr A,2000

3. Steric mass-action ion exchange: Displacement profiles and induced salt gradients;Brooks;AICHE J,1992

4. Improved isoelectric focusing chromatography on strong anion exchange media via a new model that custom designs mobile phases using simple buffers;Choy;Biotechnol Bioeng,2013

5. Theorie der Membrangleichgewichte und Membranpotentiale bei Vorhandensein von nicht dialysierenden Elektrolyten;Donnan;Zeitschrift für Elektrochemie und angewandte physikalische Chemie,1911

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