Optimized PEI-based Transfection Method for Transient Transfection and Lentiviral Production

Author:

Yang Shaozhe12,Zhou Xiaoling1,Li Rongxiang2,Fu Xiuhong2,Sun Pingnan1

Affiliation:

1. Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College; Shantou People's Republic of China

2. Reproductive and Genetic Center, The First Affiliated Hospital of Luohe Medical College; Luohe People's Republic of China

Publisher

Wiley

Subject

General Medicine

Reference28 articles.

1. Polyethyleneimine-mediated gene delivery into human adipose derived stem cells;Ahn;Biomaterials,2008

2. Nucleofection-based ex vivo nonviral gene delivery to human stem cells as a platform for tissue regeneration;Aslan;Tissue Engineering,2006

3. Current developments in gene transfection agents;Azzam;Current Drug Delivery,2004

4. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethylenimine;Boussif;Proceedings of the National Academy of Sciences of the United States of America,1995

5. Peptide- and polymer-based gene delivery vehicles;Brokx;Methods in Molecular Medicine,2004

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