Construction of Magnetic Core–Large Mesoporous Satellite Immunosensor for Long‐Lasting Chemiluminescence and Highly Sensitive Tumor Marker Determination

Author:

Liu Shude1,Li Juan12,Zou Yidong1,Jiang Yongjian3,Wu Limin4,Deng Yonghui1ORCID

Affiliation:

1. Department of Chemistry Department of Gastroenterology Zhongshan Hospital of Fudan University State Key Laboratory of Molecular Engineering of Polymers Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, iChEM Fudan University Shanghai 200433 China

2. Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine Chongqing Medical University Chongqing 400016 China

3. Department of Pancreatic Surgery Nephrology & Radiology Huashan Hospital Fudan University Shanghai 200040 China

4. Institute of Energy and Materials Chemistry Inner Mongolia University Hohhot 010021 China

Abstract

AbstractChemiluminescence immunoassay exhibits high sensitivity and signal‐to‐noise ratio, thus attracting great attention in the early diagnosis and dynamic monitoring of diseases. However, the collection of conventional flash‐type chemiluminescence signal (<5 s) relies heavily on automatic sampling and reading instrument. Herein, a novel core‐satellite multifunctional chemiluminescence immunosensor is designed for the efficient enrichment and highly sensitive determination of cancer biomarker carcinoembryonic antigen (CEA) with enhanced and long‐lasting output signal that can be conveniently recorded by a simple microplate plate reading instrument. Anti‐CEA monoclonal antibody 2 (Ab2) modified Fe3O4@SiO2 microspheres (Fe3O4@SiO2‐Ab2, 370 nm in diameter) are synthesized as the core for selectively capturing and enriching target CEA in solution, and anti‐human CEA monoclonal antibody 1 (Ab1) and horseradish peroxidase (HRP) co‐immobilized dendritic large‐mesoporous silica nanospheres (MSNs‐HRP/Ab1, 80 nm in diameter, pore size: 17 nm) are synthesized as the satellite for efficient immunological recognition and signal amplification. The as‐designed core‐satellite magnetic chemiluminescence immunosensors exhibit a broad linear range of 0.01−20 ng mL−1 and a low detection limit of 3.0 pg mL−1 for the convenient, highly specific, and sensitive determination of CEA in human serum. Such core‐satellite chemiluminescence immunosensors are expected to act as a powerful tool for in vitro detection of various biomarkers, overcome the defect of conventional chemiluminescence relying heavily on expensive and bulky automatic instruments and popularize chemiluminescence analysis to primary medical institutions and remote areas.

Funder

National Science Foundation

Fundamental Research Funds for the Central Universities

Publisher

Wiley

Subject

Biomaterials,Biotechnology,General Materials Science,General Chemistry

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