Comparison of Volumetric and Bead‐Based Counting of CD34 Cells by Single‐Platform Flow Cytometry

Author:

Saraiva Luisa1,Wang Lili2,Kammel Martin3,Kummrow Andreas3,Atkinson Eleanor4,Lee Ji Youn5,Yalcinkaya Burhanettin6,Akgöz Muslum6,Höckner Jana7,Ruf Andreas7,Engel Andrea8,Zhang Yu‐Zhong9,O'Shea Orla10,Sassi Maria Paola11,Divieto Carla11,Lekishvili Tamara12,Campbell Jonathan12,Liu Yingying13,Wang Jing13,Stebbings Richard1,Gaigalas Adolfas K.2,Rigsby Peter4,Neukammer Jörg3,Vessillier Sandrine1

Affiliation:

1. Biotherapeutics groupNational Institute for Biological Standards and Control (NIBSC) Blanche Lane, South Mimms, Potters Bar, Hertfordshire, EN6 3QG UK

2. Biosystems and Biomaterials DivisionNational Institute of Standards and Technology (NIST) Gaithersburg Maryland 20899 United States of America

3. Division of Medical Physics and Metrological Information TechnologyPhysikalisch‐Technische Bundesanstalt (PTB) Berlin, 10587 Germany

4. BiostatisticsNational Institute for Biological Standards and Control (NIBSC) Blanche Lane, South Mimms, Potters Bar, Hertfordshire, EN6 3QG UK

5. Center of LengthKorea Research Institute of Standards and Science (KRISS) Daejeon Korea

6. TUBITAK UME (National Metrology Institute of Turkey) Gebze Turkey

7. Department of Transfusion Medicine, Staedtisches Klinikum KarlsruheAcademic Hospital of the University of Freiburg Freiburg Germany

8. Becton Dickinson, BD Life Sciences Tullastrasse 8‐12, 69126, Heidelberg Germany

9. Protein and Cell Analysis, Thermo Fisher Scientific Waltham Massachusetts USA

10. UK Stem Cell Bank UK

11. Istituto Nazionale di Ricerca Metrologica (INRIM) Torino TO Italy

12. Health Science & Innovation. LGC Ltd. Queens Road Teddington, Middlesex, TN11 0LY UK

13. National Institute of Metrology (NIM) Beijing Shi China

Publisher

Wiley

Subject

Cell Biology,Histology,Pathology and Forensic Medicine

Reference14 articles.

1. The ISHAGE Guidelines for CD34+ Cell Determination by Flow Cytometry

2. Impact of standardization on clinical cell analysis by flow cytometry;Keeney M;J Biol Regul Homeost Agents,2004

3. Direct Measurement of CD34 + Blood Stem Cell Absolute Counts by Flow Cytometry

4. Procedures for detecting outlying observations in samples;Grubbs FE;Technometrics,1969

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