GONAD: A Novel CRISPR/Cas9 Genome Editing Method that Does Not Require Ex Vivo Handling of Embryos-Reference-Cited by-同舟云学术

GONAD: A Novel CRISPR/Cas9 Genome Editing Method that Does Not Require Ex Vivo Handling of Embryos

Published:2016-01 Issue:1 Volume:88 Page:
ISSN:1934-8266
Container-title:Current Protocols in Human Genetics
language:en
Short-container-title:Current Protocols in Human Genetics

Author:

Gurumurthy Channabasavaiah B.¹²,Takahashi Gou³⁴²,Wada Kenta⁴,Miura Hiromi³,Sato Masahiro⁵,Ohtsuka Masato³

Affiliation:

1. Mouse Genome Engineering Core Facility, Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center Omaha Nebraska

2. These authors contributed equally to this work

3. Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine Isehara Kanagawa Japan

4. Department of Bioproduction, Tokyo University of Agriculture Abashiri Hokkaido Japan

5. Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University Kagoshima Japan

Publisher

Wiley

Subject

General Medicine

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/0471142905.hg1508s88

Reference13 articles.

1. Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice

2. R. Behringer M. Gertsenstein K. V. Nagy A. Nagy 2014 Cold Spring Harbor Laboratory Press

3. Mouse Genome Editing Using the CRISPR/Cas System

4. Validation of microinjection methods for generating knockout mice by CRISPR/Cas‐mediated genome engineering;Horii T.;Sci. Rep.,2014

5. Simple knockout by electroporation of engineered endonucleases into intact rat embryos

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