Optimization of PCR Conditions for Amplification of GC-RichEGFRPromoter Sequence-Reference-Cited by-同舟云学术

Optimization of PCR Conditions for Amplification of GC-RichEGFRPromoter Sequence

Published:2013-11 Issue:6 Volume:27 Page:487-493
ISSN:0887-8013
Container-title:Journal of Clinical Laboratory Analysis
language:en
Short-container-title:J. Clin. Lab. Anal.

Author:

Obradovic Jasmina¹²,Jurisic Vladimir¹,Tosic Natasa³,Mrdjanovic Jasminka⁴,Perin Branislav⁵,Pavlovic Sonja³,Djordjevic Natasa¹

Affiliation:

1. Faculty of Medical Sciences; University of Kragujevac; Kragujevac Serbia

2. Faculty of Science; Institute of Biology and Ecology; University of Kragujevac; Kragujevac Serbia

3. Institute of Molecular Genetics and Genetic Engineering; University of Belgrade; Belgrade Serbia

4. Oncology Institute of Vojvodina, Faculty of Medicine, University of Novi Sad; Sremska Kamenica Serbia

5. Institute for Pulmonary Diseases of Vojvodina; Clinic for Pulmonary Oncology; Faculty of Medicine; University of Novi Sad; Sremska Kamenica Serbia

Publisher

Wiley

Subject

Microbiology (medical),Biochemistry, medical,Medical Laboratory Technology,Clinical Biochemistry,Public Health, Environmental and Occupational Health,Hematology,Immunology and Allergy

Link

http://onlinelibrary.wiley.com/wol1/doi/10.1002/jcla.21632/fullpdf

Reference41 articles.

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3. The use of K(+)-free buffers eliminates a common cause of premature chain termination in PCR and PCR sequencing;Woodford;Nucleic Acids Res,1995

4. Localised sequence regions possessing high melting temperatures prevent the amplification of a DNA mimic in competitive PCR;McDowell;Nucleic Acids Res,1998

5. CGG repeats associated with DNA instability and chromosome fragility form structures that block DNA synthesis in vitro;Usdin;Nucleic Acids Res,1995

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