Implementing <scp>l</scp>-DNA analogs as mirrors of PCR reactant hybridization state: theoretical and practical guidelines for PCR cycle control-Reference-Cited by-同舟云学术

Implementing l-DNA analogs as mirrors of PCR reactant hybridization state: theoretical and practical guidelines for PCR cycle control

Published:2024 Issue:18 Volume:16 Page:2840-2849
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Spurlock Nicholas¹^ORCID,Gabella William E.²,Nelson Dalton J.¹^ORCID,Evans David T.¹^ORCID,Pask Megan E.¹,Schmitz Jonathan E.³,Haselton Frederick R.¹^ORCID

Affiliation:

1. Department of Biomedical Engineering, Vanderbilt University, PMB 351631, Nashville, TN, USA

2. Department of Physics and Astronomy, Vanderbilt University, Nashville, TN, USA

3. Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA

Abstract

Hybridization kinetics of DNA are explored and applied to improve accuracy of annealing in sensitive PCR reactions through l-DNA hybridization-based cycling control.

Funder

National Institutes of Health

Tennessee Foundation

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/AY/D4AY00083H

Reference26 articles.

1. Adaptive PCR Based on Hybridization Sensing of Mirror-Image l-DNA

2. Multiplexed Adaptive RT-PCR Based on L-DNA Hybridization Monitoring for the Detection of Zika, Dengue, and Chikungunya RNA

3. Detection of Single-Nucleotide Polymorphism Markers of Antimalarial Drug Resistance Directly from Whole Blood

4. Preparation and Characterization of Oligonucleotides of D- and L-2’ Deoxyuridine

5. Mirror-image DNA

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