Recombinant human lecithin-cholesterol acyltransferase Fc fusion: Analysis of N- and O-linked glycans and identification and elimination of a xylose-based O-linked tetrasaccharide core in the linker region

Author:

Spahr Chris1,Kim Justin J.2,Deng Sihong2,Kodama Paul2,Xia Zhen3,Tang Jay3,Zhang Richard3,Siu Sophia4,Nuanmanee Noi1,Estes Bram1,Stevens Jennitte1,Zhou Mingyue5,Lu Hsieng S.1

Affiliation:

1. Biologics Optimization, Therapeutic Discovery, Amgen Inc.; Thousand Oaks California 91320

2. Drug Substance Development, Amgen Inc.; Seattle Washington 98119

3. Protein Technologies, Therapeutic Discovery, Amgen Inc.; South San Francisco California 94080

4. Biologics Optimization, Therapeutic Discovery, Amgen Inc.; Seattle Washington 98119

5. Metabolic Disorders, Amgen Inc.; South San Francisco California 94080

Publisher

Wiley

Subject

Molecular Biology,Biochemistry

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2. Impact of protein glycosylation on lipoprotein metabolism and atherosclerosis;Cardiovascular Research;2020-09-04

3. Characterization and prediction of positional 4-hydroxyproline and sulfotyrosine, two post-translational modifications that can occur at substantial levels in CHO cells-expressed biotherapeutics;mAbs;2019-07-24

4. O-glycosylation of Proteins;Co and Post-Translational Modifications of Therapeutic Antibodies and Proteins;2019-03-15

5. Sulfation of Proteins and Glycoproteins;Co and Post-Translational Modifications of Therapeutic Antibodies and Proteins;2019-03-15

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