Affiliation:
1. Department of Molecular Biology & Biotechnology, College of Biotechnology CCS Haryana Agricultural University Hisar Haryana India
2. Department of Botany, Deva Nagri College CCS University Meerut Uttar Pradesh India
3. Division of Genetics and Plant Breeding Sher‐e‐Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST‐Kashmir) Srinagar Jammu and Kashmir India
4. Agricultural Biotechnology Centre, Centre for Crop & Food Innovation, Food Futures Institute Murdoch University Murdoch Western Australia Australia
5. Biophysics Unit, College of Basic Sciences & Humanities GB Pant University of Agriculture & Technology Pantnagar Uttarakhand India
6. Vice‐Chancellor’s Secretariat Mahatma Jyotiba Phule Rohilkhand University Bareilly Uttar Pradesh India
7. Stockbridge School of Agriculture University of Massachusetts Amherst MA USA
Abstract
AbstractThe grain‐filling stage inTriticum aestivum(wheat) is highly vulnerable to increasing temperature as terminal heat stress diminishes grain quality and yield. To examine the mechanism of terminal heat tolerance, we performed the biochemical and gene expression analyses using two heat‐tolerant (WH730 and WH1218) and two heat‐sensitive (WH711 and WH157) wheat genotypes. We observed a significant increase in total soluble sugar (25%–47%), proline (7%–15%), and glycine betaine (GB) (22%–34%) contents in flag leaf, whereas a decrease in grain‐filling duration, 1000‐kernel weight (8%–25%), and grain yield per plant (11%–23%) was observed under the late‐sown compared to the timely sown. The maximum content of osmolytes, including total soluble sugar, proline, and GB, was observed in heat‐tolerant genotypes compared to heat‐sensitive genotypes. The expression of 10 heat‐responsive genes associated with heat shock proteins (sHsp‐1,Hsp17, andHsfA4), flavonoid biosynthesis (F3′‐1andPAL), β‐glucan synthesis (CslF6andCslH), and xyloglucan metabolism (XTH1,XTH2, andXTH5) was studied in flag leaf exposed to different heat treatments (34, 36, 38, and 40°C) at 15 days after anthesis by quantitative real‐time polymerase chain reaction. A significant increase in the relative fold expression of these genes with increasing temperature indicated their involvement in providing heat‐stress tolerance. The high differential expression of most of the genes in heat‐tolerant genotype “WH730” followed by “WH1218” indicates the high adaptability of these genotypes to heat stress compared to heat‐sensitive wheat genotypes. Based on the previous results, “WH730” performed better in terms of maximum osmolyte accumulation, grain yield, and gene expression under heat stress.
Funder
Scheme for Promotion of Academic and Research Collaboration
Subject
Plant Science,Agronomy and Crop Science,Genetics
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献