Affiliation:
1. Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China
2. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China
3. Guangxi Sericultural Research Institute, Guangxi Zhuang Autonomous Regin, Nanning 530007, China
Abstract
Mulberry (Morus alba L.) is an economically important plant for the silk industry and has the possibility of contributing immensely to Chinese pharmacopeia because of its health benefits. Domesticated silkworms feed only on mulberry leaves, meaning that the worms’ survival depends on the mulberry tree. Mulberry production is threatened by climate change and global warming. However, the regulatory mechanisms of mulberry responses to heat are poorly understood. We performed transcriptome analysis of high-temperature-stressed (42 °C) M. alba seedlings using RNA-Seq technologies. A total of 703 differentially expressed genes (DEGs) were discovered from 18,989 unigenes. Among these, 356 were up-regulated, and 347 were down-regulated. KEGG analysis revealed that most DEGs were enriched in valine, leucine and isoleucine degradation, and in starch and sucrose metabolism, alpha-linolenic acid metabolism, carotenoid biosynthesis and galactose metabolism, among others. In addition, TFs such as the NAC, HSF, IAA1, MYB, AP2, GATA, WRKY, HLH and TCP families were actively involved in response to high temperatures. Moreover, we used RT-qPCR to confirm the expression changes of eight genes under heat stress observed in the RNA-Seq analysis. This study provides M. alba transcriptome profiles under heat stress and provides theoretical bases to researchers for better understanding mulberry heat response mechanisms and breeding heat-tolerant mulberry plants.
Funder
earmarked fund
National Key R&D Program of China
Zhenjiang Science and Technology Support Project
Crop Germplasm Resources Protection Project of the Agriculture Ministry
National Infrastructure for Crop Germplasm Resources
Subject
Microbiology (medical),Molecular Biology,General Medicine,Microbiology
Reference63 articles.
1. Qin, D., Wang, G., Dong, Z., Xia, Q., and Zhao, P. (2020). Comparative Fecal Metabolomes of Silkworms Being Fed Mulberry Leaf and Artificial Diet. Insects, 11.
2. Improvement of abiotic stress adaptive traits in mulberry (Morus spp.): An update on biotechnological interventions;Sarkar;3 Biotech,2017
3. Photosynthetic Response of Plants Under Different Abiotic Stresses: A Review;Sharma;J. Plant Growth Regul.,2020
4. Research progress on heat stress and adaptation of maize;Zhang;J. Weifang Univ.,2006
5. Identification of key genes and molecular mechanisms associated with temperature stress in lentil;Sohrabi;Gene,2021
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献