Affiliation:
1. Medicinal Chemistry Laboratory, Department of Biosciences Jamia Millia Islamia New Delhi India
2. Centre for Interdisciplinary Research in Basic Sciences Jamia Millia Islamia New Delhi India
3. Special Centre for Molecular Medicine Jawaharlal Nehru University New Delhi India
Abstract
AbstractThis study performed a comparative investigation to explore the interaction mechanisms between two potential antimalarial compounds, JMI 346 and JMI 105, and human serum albumin (HSA), a vital carrier protein responsible for maintaining important biological functions. Our aim was to assess the pharmacological efficiency of these compounds while comprehensively analyzing their impact on the dynamic behavior and overall stability of the protein. A comprehensive array of multispectroscopic techniques, including UV–Vis. spectroscopy, steady‐state fluorescence analysis, synchronous fluorescence spectroscopy, three‐dimensional fluorescence and circular dichroism spectroscopy, docking studies, and molecular dynamics simulations, were performed to probe the intricate details of the interaction between the compounds and HSA. Our results revealed that both JMI 346 and JMI 105 exhibited promising pharmacological effectiveness within the context of malaria therapy. However, JMI 346 was found to exhibit a significantly higher affinity and only minor altered impact on HSA, suggesting a more favorable interaction with the protein on the dynamic behavior and overall stability of the protein in comparison to JMI 105. Further studies can build on these results to optimize the drug–protein interaction and enable the development of more potent and targeted antimalarial treatments.
Funder
Science and Engineering Research Board
Subject
Chemistry (miscellaneous),Biophysics
Cited by
2 articles.
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