Affiliation:
1. Department of Clinical Laboratory Zigong First People's Hospital Zigong Sichuan China
2. Department of Laboratory Medicine, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital University of Electronic Science and Technology of China Chengdu Sichuan China
Abstract
AbstractThe aim of this study was to investigate the interference of lipemia on measurement of HBsAg, anti‐HBs, HBeAg, anti‐HBe, anti‐HBc, anti‐HCV, HIV Ag/Ab, and anti‐TP in serum by chemiluminescent microparticle immunoassay (CMIA) and compare lipemia removing performance between high‐speed centrifugation and Lipoclear reagent. Mixed native serum samples (NSs) and hyperlipemia serum samples (HLS) were prepared for the investigated parameters. The levels of these parameters in NS and HLS were determined by CMIA on an Abbott ARCHITECT i2000SR immunoassay analyzer. HBsAg, anti‐HBs, and anti‐TP were affected with relative bias >12.5% (acceptable limit) when the level of triacylglycerol (TG) was higher than 27.12 mmol/L in HLS. Clinically unacceptable bias were observed for HBeAg and anti‐HBe in HLS with TG higher than 40.52 mmol/L. However, anti‐HCV and HIV Ag/Ab were not interfered in severe lipemia with TG < 52.03 mmol/L. In addition, the Lipoclear reagent did not reduce the interference of lipemia with relative bias from −62.50% to −18.02%. The high‐speed centrifugation under the optimized condition of 12 000g for 10 min successfully removed the interference of lipemia with relative bias from −5.93% to 0% for HBsAg, anti‐HBs, HBeAg, anti‐HBe, anti‐HBc, and anti‐TP. To conclude, high‐speed centrifugation can be used for removing the interference of lipemia to measure HBsAg, anti‐HBs, HBeAg, anti‐HBe, anti‐HBc, and anti‐TP. Accordingly, a standardized sample preanalytical preparation of the patients and other screening participants as well as a specimen examination procedure for removing lipemia interference on the serological tests was recommended.