Fast surface floating organic droplets based dispersive liquid‐liquid microextraction for trace enrichment of multiclass pesticide residues from different fruit juice samples followed by high performance liquid chromatography–diode array detection analysis

Abstract

AbstractThis study was designed to enable the development of a simple, fast, and environmentally friendly analytical technique utilizing dispersive liquid‐liquid microextraction based on surface floating organic droplets for selective and quantitative enrichment of trace level pesticide contaminants from different fruit juice samples for subsequent detection by high performance liquid chromatography, combined with a diode array detector. The selective extraction was necessitated in order to isolate the seven multiclass pesticide residues frequently occurring in fruit juice samples. The effects of experimental parameters such as pH of sample solution, type and volume of extraction and dispersive solvents, ionic strength and extraction time were optimized. The optimized method was validated using spiked blank sample and satisfactory results for accuracy, with recoveries ranging from 87.23% to 99.45%, with %relative standard deviation between 1.37 and 8.39, precision in terms of %relative standard deviation ≤ 10.78 and linearity at concentration levels from 3 to 1500 ng/ml, which corresponded with correlation coefficients ≥ 0.998. The limits of detection and the limits of quantification were ranged from 1.3×10−2 to 5.3×10−2 and 4.2×10−2 to 1.8×10−1 μg/L, respectively. At the end, the method was successfully applied to analyze real fruit juice samples and target analytes were not detected in real samples.