Carboxypeptidase D is the only enzyme responsible for antibody C-terminal lysine cleavage in Chinese hamster ovary (CHO) cells-Reference-Cited by-同舟云学术

Carboxypeptidase D is the only enzyme responsible for antibody C-terminal lysine cleavage in Chinese hamster ovary (CHO) cells

Published:2016-04-06 Issue:10 Volume:113 Page:2100-2106
ISSN:0006-3592
Container-title:Biotechnology and Bioengineering
language:en
Short-container-title:Biotechnol. Bioeng.

Author:

Hu Zhilan¹,Zhang Henry¹,Haley Benjamin²,Macchi Frank³,Yang Feng³,Misaghi Shahram¹,Elich Joseph¹,Yang Renee³,Tang Yun³,Joly John C.¹,Snedecor Bradley R.¹,Shen Amy¹

Affiliation:

1. Department of Early Stage Cell Culture; Genentech Inc.; 1 DNA Way; South San Francisco California 94080

2. Department of Molecular Biology; Genentech Inc.; 1 DNA Way; South San Francisco California

3. Department of Analytical Development and Quality Control; Genentech Inc.; 1 DNA Way; South San Francisco California

Publisher

Wiley

Subject

Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

Link

http://onlinelibrary.wiley.com/wol1/doi/10.1002/bit.25977/fullpdf

Reference23 articles.

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2. Animal cell cultures: Recent achievements and perspectives in the production of biopharmaceuticals;Butler;Appl Microbiol Biotechnol,2005

3. C-terminal lysine processing of human immunoglobulin G2 heavy chain in vivo;Cai;Biotechnol Bioeng,2011

4. Flexibility-the guiding principle for antibody manufacturing;Carson;Nat Biotechnol,2005

5. Characterizing biological products and assessing comparability following manufacturing changes;Chirino;Nat Biotechnol,2004

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