Affiliation:
1. School of Food and Biological Engineering Hefei University of Technology Hefei China
2. Engineering Research Center of Bio‐process, Ministry of Education Hefei University of Technology Hefei China
Abstract
AbstractBackgroundAflatoxin B1 (AFB1) has been considered as a prevailing natural carcinogenic mycotoxin in various food and feedstuff products, posing serious endangers to human health. The quantitative and selective detection of AFB1 was significantly essential for food safety control.ResultsWe developed a label‐free fluorescent aptasensor using a target‐binding aptamer probe as the rolling circle amplification (RCA) primer for AFB1 detection. In the presence of AFB1, the probe preferentially combined with AFB1 rather than binding to the circular template, inhibiting the initiation of RCA process. The detection limit of the proposed method was as low as 6.6 nM, and the linear dynamic response range was four orders of magnitude wide. Besides, it revealed excellent specificity for AFB1 and high‐quality detection performance in food samples.ConclusionThe presented detection method was simple, rapid, and economical, which indicated a promising application in food safety monitoring.
Funder
National Key Research and Development Program of China
National Natural Science Foundation of China
Natural Science Foundation of Anhui Province