A Bidirectional Single‐Cell Migration and Retrieval Chip for Quantitative Study of Dendritic Cell Migration-Reference-Cited by-同舟云学术

A Bidirectional Single‐Cell Migration and Retrieval Chip for Quantitative Study of Dendritic Cell Migration

Published:2023-01-19 Issue:8 Volume:10 Page:
ISSN:2198-3844
Container-title:Advanced Science
language:en
Short-container-title:Advanced Science

Author:

Shao Ning¹^ORCID,Zhou Yufu¹²,Yao Jun³,Zhang Pengchao¹,Song Yanni¹⁴,Zhang Kai¹,Han Xin¹,Wang Bin⁵,Liu Xuewu¹

Affiliation:

1. Department of Nanomedicine Houston Methodist Research Institute Houston TX 77030 USA

2. The Third Xiangya Hospital Central South University Changsha 410008 P. R. China

3. Department of Molecular and Cellular Oncology The University of Texas MD Anderson Cancer Center Houston TX 77030 USA

4. Department of Breast Surgery Harbin Medical University Cancer Hospital Harbin 150081 P. R. China

5. Department of Genetics The University of Texas MD Anderson Cancer Center Houston TX 77030 USA

Abstract

AbstractDendritic cell (DC) migration is a fundamental step during execution of its adaptive immunity functions. Studying DC migration characteristics is critical for development of DC‐dependent allergy treatments, vaccines, and cancer immunotherapies. Here, a microfluidics‐based single‐cell migration platform is described that enables high‐throughput and precise bidirectional cell migration assays. It also allows selective retrieval of cell subpopulations that have different migratory potentials. Using this microfluidic platform, DC migration is investigated in response to different chemoattractants and inhibitors, quantitatively describe DC migration patterns and retrieve DC subpopulations of different migratory potentials for differential gene expression analysis. This platform opens an avenue for precise characterization of cell migration and potential discovery of therapeutic modulators.

Funder

National Institutes of Health

Publisher

Wiley

Subject

General Physics and Astronomy,General Engineering,Biochemistry, Genetics and Molecular Biology (miscellaneous),General Materials Science,General Chemical Engineering,Medicine (miscellaneous)

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/advs.202204544

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