Affiliation:
1. Institute of Microbiology Czech Academy of Sciences Prague 14220 Czech Republic
2. Institute of Analytical Chemistry Czech Academy of Sciences Brno 602 00 Czech Republic
3. Center of Molecular Structure Institute of Biotechnology Czech Academy of Sciences Vestec 25250 Czech Republic
Abstract
AbstractThe study aims to compare different approaches and efficacies during the biological production of nanoparticles (NPs). Gold nanoparticles (AuNPs) are produced by Fusarium oxysporum at two different temperatures. One flask is incubated at 37 °C (“Common”) and the other is directly heated for 5 min at 80 °C (“Heat‐treated”). Obtained AuNPs are analyzed and compared by spectrophotometry, transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy dispersive X‐ray spectroscopy (EDS), dynamic light scattering (DLS), and Fourier transform infrared spectroscopy (FTIR). Graphite furnace atomic absorption spectroscopy (GF‐AAS) is used to determine the particle concentration after the AuNPs production. The AuNPs prepared by both (the Common and the Heat‐treated) methods exhibit maximum absorption peaks at 541 and 528 nm, respectively, and have round shapes and sizes of less than 50 nm. Their zeta potential is about −28 mV. GF‐AAS shows that the efficiency of AuNP production in Common‐ and Heat‐treated samples is equal, between 65% and 68%. Since the Heat‐treated sample shows a better size distribution, the use of higher temperature and shorter time period is preferable for the bioproduction of AuNPs. It seems that shortening the time for the production of AuNPs prevents the formation of larger NPs.
Funder
Ministerstvo Školství, Mládeže a Tělovýchovy
Subject
Condensed Matter Physics,General Materials Science,General Chemistry