Affiliation:
1. Department of Chemical Science and Engineering, Graduate School of Engineering Kobe University, Nada‐ku Kobe Hyogo Japan
2. Center for Sustainable Resource Science Tsurumi‐ku Yokohama Japan
3. Graduate School of Science Technology and Innovation Kobe University Kobe Hyogo Japan
4. PRESTO, Japan Science and Technology Agency (JST) Kawaguchi Saitama Japan
Abstract
AbstractThe demand for the essential commodity chemical 1,2‐propanediol (1,2‐PDO) is on the rise, as its microbial production has emerged as a promising method for a sustainable chemical supply. However, the reliance of 1,2‐PDO production in Escherichia coli on anaerobic conditions, as enhancing cell growth to augment precursor availability remains a substantial challenge. This study presents glucose‐based aerobic production of 1,2‐PDO, with xylose utilization facilitating cell growth. An engineered strain was constructed capable of exclusively producing 1,2‐PDO from glucose while utilizing xylose to support cell growth. This was accomplished by deleting the gloA, eno, eda, sdaA, sdaB, and tdcG genes for 1,2‐PDO production from glucose and introducing the Weimberg pathway for cell growth using xylose. Enhanced 1,2‐PDO production was achieved via yagF overexpression and disruption of the ghrA gene involved in the 1,2‐PDO‐competing pathway. The resultant strain, PD72, produced 2.48 ± 0.15 g L−1 1,2‐PDO with a 0.27 ± 0.02 g g−1‐glucose yield after 72 h cultivation. Overall, this study demonstrates aerobic 1,2‐PDO synthesis through the isolation of the 1,2‐PDO synthetic pathway from the tricarboxylic acid cycle.
Funder
Japan Science and Technology Corporation