Improving Antibody Production in Stably Transfected CHO Cells by CRISPR‐Cas9‐Mediated Inactivation of Genes Identified in a Large‐Scale Screen with Chinese Hamster‐Specific siRNAs-Reference-Cited by-同舟云学术

Improving Antibody Production in Stably Transfected CHO Cells by CRISPR‐Cas9‐Mediated Inactivation of Genes Identified in a Large‐Scale Screen with Chinese Hamster‐Specific siRNAs

Published:2020-11-09 Issue:3 Volume:16 Page:2000267
ISSN:1860-6768
Container-title:Biotechnology Journal
language:en
Short-container-title:Biotechnol. J.

Author:

Lin Pao‐Chun¹,Liu Ren²,Alvin Krista²,Wahyu Shahreel¹,Murgolo Nicholas³,Ye Jianxin²,Du Zhimei²,Song Zhiwei¹^ORCID

Affiliation:

1. Bioprocessing Technology Institute, Agency for Science Technology and Research (A*STAR) 20 Biopolis Way, #04‐01 Centros Singapore 138668 Singapore

2. Cell Line Development, MRL Merck & Co., Inc. 2000 Galloping Hill Road Kenilworth NJ 07033 USA

3. Bioinformatics, MRL Merck & Co., Inc. 2000 Galloping Hill Road Kenilworth NJ 07033 USA

Publisher

Wiley

Subject

Molecular Medicine,Applied Microbiology and Biotechnology,General Medicine

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/biot.202000267

Reference41 articles.

1. CHO cells in biotechnology for production of recombinant proteins: current state and further potential

2. 25 years of recombinant proteins from reactor-grown cells — Where do we go from here?

3. Guidelines to cell engineering for monoclonal antibody production

4. Advances in Mammalian Cell Line Development Technologies for Recombinant Protein Production

5. Production platforms for biotherapeutic glycoproteins. Occurrence, impact, and challenges of non-human sialylation

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