Studying the impact of cell age on the yeast growth behaviour of Saccharomyces pastorianus var. carlsbergensis by magnetic separation

Author:

Eigenfeld Marco1ORCID,Wittmann Leonie2ORCID,Kerpes Roland1ORCID,Schwaminger Sebastian P.234ORCID,Becker Thomas1ORCID

Affiliation:

1. TUM School of Life Science Technical University of Munich Chair of Brewing and Beverage Technology Freising Germany

2. TUM School of Engineering and Design Technical University of Munich Chair of Bioseparation Engineering Garching Germany

3. Otto‐Loewi Research Center Division of Medicinal Chemistry Medical University of Graz Graz Austria

4. BioTechMed‐Graz Graz Austria

Abstract

AbstractDespite the fact that yeast is a widely used microorganism in the food, beverage, and pharmaceutical industries, the impact of viability and age distribution on cultivation performance has yet to be fully understood. For a detailed analysis of fermentation performance and physiological state, we introduced a method of magnetic batch separation to isolate daughter and mother cells from a heterogeneous culture. By binding functionalised iron oxide nanoparticles, it is possible to separate the chitin‐enriched bud scars by way of a linker protein. This reveals that low viability cultures with a high daughter cell content perform similarly to a high viability culture with a low daughter cell content. Magnetic separation results in the daughter cell fraction (>95%) showing a 21% higher growth rate in aerobic conditions than mother cells and a 52% higher rate under anaerobic conditions. These findings emphasise the importance of viability and age during cultivation and are the first step towards improving the efficiency of yeast‐based processes.

Funder

Deutsche Forschungsgemeinschaft

Publisher

Wiley

Subject

Molecular Medicine,Applied Microbiology and Biotechnology,General Medicine

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