Advances in serum‐free media for CHO cells: From traditional serum substitutes to microbial‐derived substances

Author:

Zhang Mingcan12,Zhao Xinyu1,Li Ying1,Ye Qinghua1,Wu Yuwei1,Niu Qinya12,Zhang Ying12,Fan Guanghan12,Chen Tianxiang12,Xia Jiarui12,Wu Qingping12ORCID

Affiliation:

1. National Health Commission Science and Technology Innovation Platform for Nutrition and Safety of Microbial Food, Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology Guangdong Academy of Sciences Guangzhou China

2. Department of Food Science and Technology, School of Agriculture and Biology Shanghai Jiao Tong University Shanghai China

Abstract

AbstractThe Chinese hamster ovary (CHO) cell is an epithelial‐like cell that produces proteins with post‐translational modifications similar to human glycosylation. It is widely used in the production of recombinant therapeutic proteins and monoclonal antibodies. Culturing CHO cells typically requires the addition of a certain proportion of fetal bovine serum (FBS) to maintain cell proliferation and passaging. However, serum is characterized by its complex composition, batch‐to‐batch variability, high cost, and potential risk of exogenous contaminants such as mycoplasma and viruses, which impact the purity and safety of the synthesized proteins. Therefore, search for serum alternatives and development of serum‐free media for CHO‐based protein biomanufacturing are of great significance. This review systematically summarizes the application advantages of CHO cells and strategies for high‐density expression. It highlights the developmental trends of serum substitutes from human platelet lysates to animal‐free extracts and microbial‐derived substances and elucidates the mechanisms by which these substitutes enhance CHO cell culture performance and recombinant protein production, aiming to provide theoretical guidance for exploring novel serum alternatives and developing serum‐free media for CHO cells.

Funder

National Key Research and Development Program of China

Natural Science Foundation of Guangdong Province

Publisher

Wiley

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3