Single Molecule with a Large Transistor – SiMoT cytokine IL‐6 Detection Benchmarked against a Chemiluminescent Ultrasensitive Immunoassay Array

Author:

Scandurra Cecilia12,Björkström Kim3,Sarcina Lucia1,Imbriano Anna12,Di Franco Cinzia1,Österbacka Ronald3,Bollella Paolo12,Scamarcio Gaetano4,Torsi Luisa123ORCID,Macchia Eleonora235ORCID

Affiliation:

1. Dipartimento di Chimica Università degli Studi di Bari “Aldo Moro” Bari 70125 Italy

2. Centre for Colloid and Surface Science – CSGI Unit of University of Bari Università degli Studi di Bari “Aldo Moro” Bari 70125 Italy

3. The Faculty of Science and Engineering Åbo Akademi University Turku 20500 Finland

4. Dipartimento Interateneo di Fisica Università degli Studi di Bari Aldo Moro Bari 70125 Italy

5. Dipartimento di Farmacia‐Scienze del Farmaco Università degli Studi di Bari “Aldo Moro” Bari 70125 Italy

Abstract

AbstractEarly diagnosis and efficient treatments of oncological, neurological, inflammatory, and infectious diseases rely more and more on ultrasensitive detection of protein markers; the ultimate limit is a reliable immunoassay capable of single‐protein detection. Among protein biomarkers, cytokines play a key role in clinical diagnosis as they are involved in developing many complex diseases and disorders, such as chronic inflammatory diseases including metabolic syndrome, neurodegenerative diseases, and cardiovascular diseases, along with autoimmune diseases and cancer. Herein, the improvement of a Single Molecule with Transistor (SiMoT) is reported based on an electrolyte‐gated organic field‐effect transistor applied for the detection of cytokine IL‐6 in blood serum, reaching a limit‐of‐detection (LOD) of 1 ± 1 protein in a sample of 0.1 mL. The analytical performance levels are benchmarked against the Simoa Planar Array SP‐X technology, a benchtop chemiluminescent array. After comprehensive optimization, Simoa SP‐X by using a multivariate experimental design approach exhibits a LOD 103 higher than SiMoT. The proposed SiMoT electronic assay is label‐free, fast (30 min), and selective, paving the way for an ultra‐sensitive point‐of‐care immunoassay platform enabling pre‐symptomatic disease diagnosis.

Funder

Horizon 2020 Framework Programme

Publisher

Wiley

Subject

Industrial and Manufacturing Engineering,Mechanics of Materials,General Materials Science

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