Affiliation:
1. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei MOST) & Key Laboratory of Oral Biomedicine Ministry of Education School & Hospital of Stomatology Wuhan University Wuhan 430079 P. R. China
2. Medical Research Institute School of Medicine Wuhan University Wuhan 430071 P. R. China
3. TaiKang Center for Life and Medical Sciences Wuhan University Wuhan 430071 P. R. China
Abstract
AbstractThe oral cavity comprises an environment full of microorganisms. Dysregulation of this microbial‐cellular microenvironment will lead to a series of oral diseases, such as implant‐associated infection caused by Staphylococcus aureus (S. aureus) biofilms and periodontitis initiated by Streptococcus oralis (S. oralis). In this study, a liposome‐encapsulated indocyanine green (ICG) and rapamycin drug‐delivery nanoparticle (ICG‐rapamycin) is designed to treat and prevent two typical biofilm‐induced oral diseases by regulating the microbial‐cellular microenvironment. ICG‐rapamycin elevates the reactive oxygen species (ROS) and temperature levels to facilitate photodynamic and photothermal mechanisms under near‐infrared (NIR) laser irradiation for anti‐bacteria. In addition, it prevents biofilm formation by promoting bacterial motility with increasing the ATP levels. The nanoparticles modulate the microbial‐cellular interaction to reduce cellular inflammation and enhance bacterial clearance, which includes promoting the M2 polarization of macrophages, upregulating the anti‐inflammatory factor TGF‐β, and enhancing the bacterial phagocytosis of macrophages. Based on these findings, ICG‐rapamycin is applied to implant‐infected and periodontitis animal models to confirm the effects in vivo. This study demonstrates that ICG‐rapamycin can treat and prevent biofilm‐induced oral diseases by regulating the microbial‐cellular microenvironment, thus providing a promising strategy for future clinical applications.
Funder
National Science Fund for Distinguished Young Scholars
National Natural Science Foundation of China
Fundamental Research Funds for the Central Universities
Subject
Mechanical Engineering,Mechanics of Materials,General Materials Science
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献