Breaking the Photostability and pH Limitation of Halo‐Fluoresceins through Chitosan Conjugation

Author:

Wang Ying1,Lang Yunhe1,Yang Qin2,Wu Peng1ORCID

Affiliation:

1. Analytical and Testing Centre State Key Laboratory of Hydraulics and Mountain River Engineering Sichuan University Chengdu 610064 China

2. College of Biomass Science and Engineering Sichuan University Chengdu 610064 China

Abstract

AbstractHalo‐fluoresceins are widely used in cell and tissue staining, intracellular sensing, and photodynamic therapy, but their notorious photo‐instability and pH dependence restrict their applications, especially in long‐term visible light exposure and acidic environments. To overcome these limitations, here a strategy is proposed of conjugating chitosan with the carboxyl group of halo‐fluorescein (CS‐halofluorescein). The cross‐linked polymer chains and the hydrogen‐bonding networks of chitosan help shielding out 1O2 from direct attacking the encapsulated halo‐fluoresceins, leading to a two orders of magnitude lower photobleaching rate. Meanwhile, the condensation of primary amines of chitosan with the carboxyl group on halo‐fluorescein blocks the pH‐dependent intramolecular spirocyclization, leading to pH‐inert fluorescein derivatives. The greatly improved photostability and pH inertness of CS‐halofluoresceins can be harvested for aerobic photoredox synthesis and photodynamic bacteria inactivation in extremely acidic media. Moreover, food additive nature of chitosan and erythrosine (TIF) and excellent film‐forming property of chitosan allow coating‐based light‐assisted preservation of perishable fruits, leading to appreciably extended shelf life of fruits (e.g., perishable strawberry, rt: > 3 days; 4 °C: > 5 days).

Funder

National Natural Science Foundation of China

Sichuan University

Publisher

Wiley

Subject

Mechanical Engineering,Mechanics of Materials,General Materials Science

Reference54 articles.

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