Affiliation:
1. School and Hospital of Stomatology Fujian Medical University Fuzhou China
2. NHC Key Laboratory of Diagnosis and Therapy of Gastrointestinal Tumor & Key Laboratory of Molecular Diagnostics and Precision Medicine for Surgical Oncology in Gansu Province Gansu Provincial Hospital Lanzhou China
3. Key Laboratory of Radiation Technology and Biophysics, Hefei Institute of Physical Science Chinese Academy of Sciences Hefei China
4. Jiangxi Health‐Biotech Stem Cell Technology Co., Ltd. Jiangxi Research Center of Stem Cell Engineering Shangrao China
Abstract
AbstractSupernumerary teeth are advantaged sources for high‐quality stem cell preparation from both apical papilla (SCAP‐Ss) and dental pulp (DPSCs). However, the deficiency of the systematic and detailed comparison of the biological and transcriptomic characteristics of the aforementioned stem cells largely hinders their application in regenerative medicine. Herein, we collected supernumerary teeth for SCAP‐S and DPSC isolation and identification by utilizing multiple biological tests (e.g., growth curve, cell cycle and apoptosis, adipogenic and osteogenic differentiation, and quantitative real‐time polymerase chain reaction). Furthermore, we took advantage of transcriptome sequencing and multifaceted bioinformatic analyses to dissect the similarities and diversities between them. In this study, we found that SCAP‐Ss and DPSCs showed indistinctive signatures in morphology and immunophenotypes, whereas with diversity in cell vitality and multi‐lineage differentiation as well as gene expression profiling and differentially expressed genes‐associated gene ontology and signaling pathways. Collectively, our data indicated the diversity of the multifaceted signatures of human supernumerary teeth‐derived stem cells both at the cellular and molecular levels, which also supplied new references for SCAP‐Ss serving as splendid alternative stem cell sources for regenerative medicine purposes.
Funder
Natural Science Foundation of Fujian Province
Natural Science Foundation of Jiangxi Province
National Natural Science Foundation of China
Subject
Cell Biology,General Medicine