Assurance of monoclonality in one round of cloning through cell sorting for single cell deposition coupled with high resolution cell imaging

Author:

Evans Krista1,Albanetti Thomas1,Venkat Raghavan1,Schoner Ronald1,Savery James2,Miro‐Quesada Guillermo2,Rajan Bhargavi3,Groves Christopher3

Affiliation:

1. Cell Culture and Fermentation Sciences, Biopharmaceutical DevelopmentMedImmune, Gaithersburg MD 20878

2. Data Management and Quantitative SciencesMedImmune, Gaithersburg MD 20878

3. Respiratory, Inflammation and AutoimmunityMedImmune, Gaithersburg MD 20878

Funder

MedImmune

Publisher

Wiley

Subject

Biotechnology

Reference15 articles.

1. Selection methods for high-producing mammalian cell lines

2. International Conference on Harmonization.ICH Harmonised Tripartite Guideline: Derivation and Characterization of Cell Substrates used for Production of Biotechnological/Biological Products Q5D. International Conference on Harmonization; 1997. Published Federal Register. 1998; 63.182:50244-50249. Available at:http://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Quality/Q5D/Step4/Q5D_Guideline.pdf. Accessed May 15 2014.

3. Center for Biologics Evaluation and Research (CBER) U.S.Supplement to the Points to Consider in the Production and Testing of New Drugs and Biologic Produced by Recombinant DNA Technology: Nucleic Acid Characterization and Genetic Stability. U.S. Food and Drug Administration; 1992. Available at:http://www.fda.gov/downloads/biologicsbloodvaccines/guidancecomplianceregulatory information/otherrecommendationsformanufacturers/ucm062777.pdf. Accessed May 15 2014.

4. World Health Organization.Guidelines on the Quality Safety and Efficacy of Biotherapeutic Protein Products Prepared by Recombinant DNA Technology. Annex 3 in WHO Expert Committee on Biological Standardization.Sixty‐First Report. WHO Technical Report Series No. 978. WHO Press World Health Organization; 2013. Available at:http://www.who.int/biologicals/expert_committee/TRS_978_61st_report.pdf. Accessed May 15 2014.

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