Affiliation:
1. Department of Food Science and Nutrition School of Human Science and Environment University of Hyogo 1‐1‐12, Shinzaike‐Honcho Himeji Hyogo 670‐0092 Japan
2. Research Institute for Food and Nutritional Sciences University of Hyogo 1‐1‐12, Shinzaike‐Honcho Himeji Hyogo 670‐0092 Japan
3. Department of Biochemistry and Applied Biosciences Faculty of Agriculture University of Miyazaki Gakuen‐kibanadai‐nishi‐1‐1 Miyazaki 889‐2192 Japan
Abstract
ScopeQuercetin (QUE), a phytochemical found in various plant foods, has been shown to have a variety of physiological activities in vivo, though biological sites where it has activities and the mechanisms of transport have not been fully elucidated.Methods and resultsIn the present study, intracellular uptake of QUE into HT‐29 human colon adenocarcinoma cells is found to result in spontaneous release of extracellular vesicles (EVs), which are subsequently embedded with QUE. In addition, QUE‐embedded EVs are detected in serum of QUE‐administered Sprague‐Dawley rats. Interestingly, the rate of cellular uptake of QUE‐encapsulated EVs (EV‐QUE) into RAW264.7 macrophages is markedly higher than that of free QUE. Moreover, EV‐QUE suppresses lipopolysaccharide (LPS)‐induced nitric oxide at a lower concentration than free QUE.ConclusionThe present findings suggest that QUE may be embedded in EVs in the gastrointestinal tract, then become absorbed and enter the bloodstream to exhibit biological activities.
Funder
Urakami Foundation for Food and Food Culture Promotion
Subject
Food Science,Biotechnology