Affiliation:
1. Faculty of Dentistry National University of Singapore Singapore 119085 Singapore
2. Singapore Institute of Manufacturing Technology (SIMTech) Agency for Science, Technology and Research (A*STAR) Singapore 138634 Singapore
3. REVIVO BioSystems Pte. Ltd. Singapore 138623 Singapore
4. ORCHIDS: Oral Care Health Innovations and Designs Singapore National University of Singapore Singapore 119085 Singapore
5. NUS Centre for Additive Manufacturing (AM.NUS) National University of Singapore Singapore 117602 Singapore
Abstract
AbstractGingiva plays a crucial barrier role at the interface of teeth, tooth‐supporting structures, microbiome, and external agents. To mimic this complex microenvironment, an in vitro microphysiological platform and biofabricated full‐thickness gingival equivalents (gingiva‐on‐chip) within a vertically stacked microfluidic device is developed. This design allowed long‐term and air‐liquid interface culture, and host‐material interactions under flow conditions. Compared to static cultures, dynamic cultures on‐chip enabled the biofabrication of gingival equivalents with stable mucosal matrix, improved epithelial morphogenesis, and barrier features. Additionally, a diseased state with disrupted barrier function representative of gingival/oral mucosal ulcers is modeled. The apical flow feature is utilized to emulate the mechanical action of mouth rinse and integrate the assessment of host‐material interactions and transmucosal permeation of oral‐care formulations in both healthy and diseased states. Although the gingiva‐on‐chip cultures have thicker and more mature epithelium, the flow of oral‐care formulations induced increased tissue disruption and cytotoxic features compared to static conditions. The realistic emulation of mouth rinsing action facilitated a more physiological assessment of mucosal irritation potential. Overall, this microphysiological system enables biofabrication of human gingiva equivalents in intact and ulcerated states, providing a miniaturized and integrated platform for downstream host‐material and host‐microbiome applications in gingival and oral mucosa research.
Funder
National University of Singapore
Singapore-MIT Alliance for Research and Technology Centre
Agency for Science, Technology and Research
National University Health System
Subject
Pharmaceutical Science,Biomedical Engineering,Biomaterials
Cited by
2 articles.
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