Affiliation:
1. School of Chemistry University of New South Wales Sydney 2052 Australia
2. Institut für Chemie und Biochemie Freie Universität Berlin Fabeckstraße 34–36 14195 Berlin Germany
3. EMBL Australia Node for Single Molecule Science, School of Biomedical Sciences University of New South Wales Sydney 2052 Australia
4. ARC Centre of Excellence in Synthetic Biology University of New South Wales Sydney 2052 Australia
Abstract
AbstractHierarchical DNA nanostructures offer programmable functions at scale, but making these structures dynamic, while keeping individual components intact, is challenging. Here we show that the DNA A‐motif—protonated, self‐complementary poly(adenine) sequences—can propagate DNA origami into one‐dimensional, micron‐length fibrils. When coupled to a small molecule pH regulator, visible light can activate the hierarchical assembly of our DNA origami into dissipative fibrils. This system is recyclable and does not require DNA modification. By employing a modular and waste‐free strategy to assemble and disassemble hierarchical structures built from DNA origami, we offer a facile and accessible route to developing well‐defined, dynamic, and large DNA assemblies with temporal control. As a general tool, we envision that coupling the A‐motif to cycles of dissipative protonation will allow the transient construction of diverse DNA nanostructures, finding broad applications in dynamic and non‐equilibrium nanotechnology.
Funder
Australian Research Council
Subject
General Chemistry,Catalysis