Intracellular Molecules Induced Extracellular Peptide Self‐Assembly for Efficient and Effective In Situ Cell Purification

Author:

Li Cheuk Yin1,Liang Zhenghua1ORCID,Liu Lejian2,Kuang Yi1ORCID

Affiliation:

1. Department of Chemical and Biological Engineering The Hong Kong University of Science and Technology Clear Water Bay, Kowloon Hong Kong Hong Kong

2. Department of Biomedical Engineering The Hong Kong Polytechnic University Hung Hom, Kowloon Hong Kong Hong Kong

Abstract

AbstractSynthetic messenger RNA (mRNA) switches are powerful tools for in situ cell purification, especially for cells derived from stem cells. However, the retention effectiveness of the target cells is limited by the leaky expression of toxic protein. The elimination efficiency of non‐target cells is also constrained due to the lack of signal amplification. In this study, we designed a novel approach that uses synthetic mRNA switch to convey intracellular marker molecule information into spatially controlled extracellular toxic assembly formation. The approach bypasses the use of toxic protein to ensure high target cell recovery effectiveness. Meanwhile, the marker molecule information is amplified at multiple levels to ensure high non‐target cell elimination effectiveness. Our approach can be tailored to meet various in situ cell purification needs, promising high‐quality in situ cell purification for a wide range of biomedical applications.

Publisher

Wiley

Subject

General Chemistry,Catalysis

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