A DNA Logic Circuit Equipped with a Biological Amplifier Loaded into Biomimetic ZIF‐8 Nanoparticles Enables Accurate Identification of Specific Cancers In Vivo

Author:

Wei Kaiji1ORCID,He Mengyun1,Zhang Juan1,Zhao Chuan1,Nie Cunpeng1,Zhang Tong1,Liu Yi1,Chen Tingting1,Jiang Jianhui1,Chu Xia1ORCID

Affiliation:

1. State Key Laboratory of Chemo/Bio-Sensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University Changsha 410082 China

Abstract

AbstractDNA logic circuits (DLC) enable the accurate identification of specific cell types, such as cancer cells, but they face the challenges of weak output signals and a lack of competent platforms that can efficiently deliver DLC components to the target site in the living body. To address these issues, we rationally introduced a cascaded biological amplifier module based on the Primer Exchange Reaction inspired by electronic circuit amplifier devices. As a paradigm, three abnormally expressed Hela cell microRNAs (‐30a, ‐17, and ‐21) were chosen as “AND” gate inputs. DLC response to these inputs was boosted by the amplifier markedly enhancing the output signal. More importantly, the encapsulation of DLC and amplifier components into ZIF‐8 nanoparticles resulted in their efficient delivery to the target site, successfully distinguishing the Hela tumor subtype from other tumors in vivo. Thus, we envision that this strategy has great potential for clinical cancer diagnosis.

Funder

National Natural Science Foundation of China

National Key Research and Development Program of China

Department of Science and Technology of Hunan Province

Natural Science Foundation of Hunan Province

Publisher

Wiley

Subject

General Chemistry,Catalysis

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