Trehalose Delays Senescence in Cut Gladiolus Spikes

Abstract

Florets of cut gladiolus (Gladiolus ×grandiflora hort. cv. Fujinoyuki) spikes kept at 25 °C under 14 h light/10 h dark condition showed severe wilting 4 days after flower opening. Treatment with 0.1 m trehalose prolonged vase-life 2 days, whereas inhibitors and other sugars had no effect. The upper florets also opened properly in trehalose-treated spikes, but not in cycloheximide-treated ones. After 4 days, the first florets of trehalose-treated spikes maintained water content more effectively than did controls or spikes treated with other sugars. The parenchyma adjacent to vascular bundles in the petals of trehalose-treated spikes maintained viability for 4 days. These results suggest that trehalose preserves cell viability in gladiolus spikes, thereby enhancing water uptake into petal tissues.