Collagen Content in Skin and Internal Organs of the Tight Skin Mouse: An Animal Model of Scleroderma

Abstract

The Tight Skin mouse is a genetically induced animal model of tissue fibrosis caused by a large in-frame mutation in the gene encoding fibrillin-1 (Fbn-1). We examined the influence of gender on the collagen content of tissues in C57BL/6J wild type (+/+) and mutant Tight Skin (Tsk/+) mice employing hydroxyproline assays. Tissue sections were stained with Masson’s trichrome to identify collagenin situ. AdultTsk/+ mice skin contains ~15% more collagen, on average, than skin from +/+ mice of the same gender. The heart ofTsk/+ males had significantly more collagen than that of +/+ males. No significant gender differences were found in lungs and kidney collagen content. Overall, the collagen content ofTsk/+ males and +/+ males was higher than that of theirTsk/+ and +/+ female counterparts, respectively. Our data confirm increased deposition of collagen in skin and hearts ofTsk/+ mice; however, the effects of theTskmutation on collagen content are both tissue specific and gender specific. These results indicate that comparative studies of collagen content between normal andTsk/+ mice skin and internal organs must take into account gender differences caused by expression of the androgen receptor.