Efficient and Rapid Detection ofSalmonellaUsing Microfluidic Impedance Based Sensing

Author:

Ghosh Dastider Shibajyoti1,Barizuddin Syed2,Yuksek Nuh S.1,Dweik Majed2,Almasri Mahmoud F.1

Affiliation:

1. Department of Electrical and Computer Engineering, University of Missouri, Columbia, MO 65211, USA

2. Cooperative Research and Life & Physical Sciences, Lincoln University, Jefferson City, MO 65101, USA

Abstract

We present a low cost, easy to fabricate biosensor, which can quickly and accurately detectSalmonella typhimurium. This study also compares the advantages of the microfluidic biosensor over a nonmicrofluidic biosensor. High density interdigitated electrode array was used to detectSalmonellacells inside a microfluidic chip. Monoclonal anti-Salmonellaantibodies were allowed to be immobilized on the surface of the electrode array for selective detection ofSalmonella typhimurium. An impedance analyzer was used to measure and record the response signal from the biosensor. The biosensor provides qualitative and quantitative results in 3 hours without any enrichment steps. The microfluidic biosensor’s lower detection limit was found to be3×103 CFU/mL compared to the3×104 CFU/mL of the nonmicrofluidic biosensor, which shows that the microfluidic biosensor has 10-fold increased sensitivity. The impedance response of microfluidic biosensor was also significantly higher (2 to 2.9 times) compared to the nonmicrofluidic biosensor.

Funder

National Science Foundation

Publisher

Hindawi Limited

Subject

Electrical and Electronic Engineering,Instrumentation,Control and Systems Engineering

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