An Alternative Method for Long-Term Culture of Chicken Embryonic Stem Cell In Vitro

Author:

Zhang Li1,Wu Yenan1,Li Xiang1,Wei Shao1,Xing Yiming2,Lian Zhengxing13,Han Hongbing13ORCID

Affiliation:

1. Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China

2. State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, China

3. National Engineering Laboratory For Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China

Abstract

Chicken embryonic stem cells (cESCs) obtained from stage X embryos provide a novel model for the study of avian embryonic development. A new way to maintain cESCs for a long period in vitro still remains unexplored. We found that the cESCs showed stem cell-like properties in vitro for a long term with the support of DF-1 feeder and basic culture medium supplemented with human basic fibroblast growth factor (hbFGF), mouse stem cell factor (mSCF), and human leukemia inhibitory factor (hLIF). During the long culture period, the cESCs showed typical ES cell morphology and expressed primitive stem cell markers with a relatively stable proliferation rate and high telomerase activity. These cells also exhibited the capability to differentiate into cardiac myocytes, smooth muscle cells, neural cells, osteoblast, and adipocyte in vitro. Chimera chickens were produced by cESCs cultured for 25 passages with this new culture system. The experiments showed that DF-1 was the optimal feeder and hbFGF was an important factor for maintaining the pluripotency of cESCs in vitro.

Funder

National Natural Science Foundation of China

Publisher

Hindawi Limited

Subject

Cell Biology,Molecular Biology

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