Evaluation of Antibacterial Enrofloxacin in Eggs by Matrix Solid Phase Dispersion-Flow Injection Chemiluminescence

Abstract

The study based on the chemiluminescence (CL) reaction of potassium ferricyanide and luminol in sodium hydroxide medium, enrofloxacin (ENRO) could dramatically enhance CL intensities and incorporated with matrix solid-phase dispersion (MSPD) technique (Florisil used as dispersant, dichloromethane eluted the target compounds). A simple flow injection chemiluminescence (FL-CL) method with MSPD technique for determination of ENRO in eggs was described. Under optimal conditions, the CL intensities were linearly related to ENRO concentration ranging from4.0×10-8 g.L−1to5.0×10-5 g.L−1, with a correlation coefficient of 0.9989 and detection limit of5.0×10-9 g.L−1. The relative standard deviation was 3.6% at an ENRO concentration of2.0×10-6 g.L−1. Our testing technique can help ensure food safety, and thus, protect public health.