Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry

Author:

Freitas P. V.1,da Silva D. R.2ORCID,Beluomini M. A.2,da Silva J. L.2,Stradiotto N. R.3

Affiliation:

1. Departamento de Tecnologia de Alimentos, Centro de Ciências Exatas e Tecnológicas, Universidade Federal de Viçosa, Av. Peter Henry Rolfs s/n, Viçosa, MG, Brazil

2. Departmento de Química Analítica, Instituto de Química (IQ/CAr), Universidade Estadual Paulista, R. Prof. Francisco Degni 55, Jardim Quitandinha, 14800-060 Araraquara, SP, Brazil

3. Universidade Federal Fluminense, Escola de Engenharia Industrial e Metalúrgica de Volta Redonda, Av. dos Trabalhadores 420, Vila Santa Cecília, Volta Redonda, RJ, Brazil

Abstract

A reversed-phase liquid chromatographic separation with pulsed amperometric detection of phenolic acids at a glassy carbon electrode is described. Chromatographic separation was carried out in isocratic conditions using 0.20 mol·L−1acetic acid (pH 5.0)/water (80 : 20,v/v) as mobile phase under constant working potential mode of 0.80 V. Chromatographic peaks presented high resolution and separation. Calibration curves exhibited excellent correlation coefficients, above 0.995. Linear ranges of the analytes, in mg L−1, were of 0.018–18 (gallic acid), 0.146–19 (vanillic acid), 0.13–17 (caffeic acid), 0.016–16 (ferulic acid), and 0.008–17 (p-coumaric acid), respectively. Limits of detection ranged from 1.6 to 97 μg·L−1and precision varied in 1.73–3.78% interval. Concentrations of 19 ± 0.51 mg·L−1and 7.8 ± 2.5 mg·L−1were found for vanillic and caffeic acids, respectively, in a sugarcane vinasse sample. Gallic, ferulic, and p-coumaric acids were not detected. Recovery results demonstrated that the proposed method is accurate, and it can be used to detect and quantify phenolic acids in sugarcane vinasse without any influence of interferents.

Funder

Fundação de Amparo à Pesquisa do Estado de São Paulo

Publisher

Hindawi Limited

Subject

Computer Science Applications,Instrumentation,General Chemical Engineering,Analytical Chemistry

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3