Mycosynthesis of Noble Metal Nanoparticle Using Laetiporus versisporus Mushroom and Analysis of Antioxidant Activity

Abstract

Nanoparticles being the backbone of nanotechnology gain more attention nowadays as they are entirely different from the colloids and also exhibit unique electrochemical, physical, and optical properties corresponding to their dimensions and shape. The core concept of getting nanoparticles from metal colloids lies on the traditional approach that is applied in both conventional and mechanical methods these days. Among those, the techniques that rely on the biological systems for the generation of nanoparticles have more merits and precedence over other environmental mechanisms as this mitigates the expense as well as toxicity. Considering both nutritional and medicinal values of mushrooms, they were properly managed, adopted, and accustomed in distinct skills to get desired products of nanosizes. Gold is commonly known as a noble metal, and it owns enormous medicinal values since long time in history. Here, a mycosynthesis approach was applied; by the way, the mushroom Laetiporus versisporus and gold ions collaborate to bring out gold nanoparticles (AuNPs) were synthesized by the involvement of Laetiporus versisporus. Mycosynthesis deals with an environmentally friendly process that leads to the extracellular synthesis of AuNPs. The Laetiporus versisporus were collected from hills of Kodaikanal, Tamil Nadu. The gold source turned into ultrafiner particles which were then computed and monitored through UV-Vis spectrophotometer and Fourier-transform infrared (FTIR) spectroscopy, and the texture of particles was affirmed with the help of peaks originated in X-ray diffraction (XRD). Transmission electron microscopy (TEM) techniques and scanning electron microscope (SEM) unveiled spherical AuNPs, and the overall scale of which was figured as 10 nm. Various concentrations of AuNPs by the concentration of extracted Laetiporus versisporus were evaluated for antioxidant activity using the standard in vitro methods like ferrous ion chelating effect, nitric oxide (NO) scavenging assay, and DPPH assay activity.