Effect of Fluorosis on Liver Cells of VC Deficient and Wild Type Mice

Abstract

For decades, mouse and other rodents have been used for the study of oxidative or related studies such as the effect of fluoride. It is known that rodents normally synthesize their own vitamin C (VC) due to the presence of a key enzyme in ascorbic acid synthesis, l-gulono-lactone-γ-oxidase (Gulo), while humans do not have the capacity of VC synthesis due to the deletion of most parts of the GULO gene. The spontaneous fracture (sfx) mouse recently emerged as a model for study of VC deficiency. We investigated the effect of fluoride on liver cells from wild type Balb/c andsfxmice. We found that activities of SOD, GPx, and CAT were reduced in both wild type andsfxmice; however, the amount of reduction in thesfxcells is more than that in Balb/c cells. In addition, while both cells increased MDA, the increase in thesfxcells is greater than that in Balb/c cells. Gene networks ofSod,Gpx, andCatin the liver of humans and mice are also different. Our study suggests that reaction to fluoride in vitamin C deficient mice might be different from that of wild type mice.