Application of Factorial Design and Response Surface Methodology in the Optimization of Clindamycin Nanocomposites

Abstract

This study is aimed at achieving the optimized preparation of a new extended-release formulation of clindamycin (CLD) via loading of the CLD onto chitosan-chondroitin sulfate (CS-Chondro). The CS-Chondro-CLD nanocomposites were prepared by mixing different masses of CS (50, 100, and 200 mg) with Chondro (50, 100, and 200 mg) and different masses of CLD (75, 150, and 300 mg). The prepared nanocomposites were characterized by different techniques including loading efficiency (LE), X-ray powder diffraction (XRD), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and release study. The XRD spectra of CS-Chondro-CLD nanocomposites showed two peaks at $2\theta =22.5$ ° and 40.7°, indicating amorphous forms. The FTIR data shows incorporation of CLD into the CS-Chondro polymers. An in vitro release study of CLD from nanocomposites was carried out using PBS at pH 7.4. The result showed that the release rate was completed after 25 hours. This study showed that the CS-Chondro-CLD nanocomposites have promising applications in the delivery of CLD drug.