Preparation and Characterization of Patuletin-Loaded Chitosan Nanoparticles with Improved Selectivity and Safety Profiles for Anticancer Applications-Reference-Cited by-同舟云学术

Preparation and Characterization of Patuletin-Loaded Chitosan Nanoparticles with Improved Selectivity and Safety Profiles for Anticancer Applications

Published:2023-07-24 Issue: Volume:2023 Page:1-11
ISSN:2090-9071
Container-title:Journal of Chemistry
language:en
Short-container-title:Journal of Chemistry

Author:

Metwaly Ahmed M.¹²^ORCID,Abdel-Raoof Ahmed M.³^ORCID,Alattar Abdulaziz M.³^ORCID,El-Zomrawy Adham A.⁴^ORCID,Ashmawy Ashraf M.⁴^ORCID,Metwally Metwally G.⁵^ORCID,Abu-Saied Mohamed A.⁶^ORCID,Lotfy Asmaa M.⁶^ORCID,Alsfouk Bshra A.⁷^ORCID,Elkaeed Eslam B.⁸^ORCID,Eissa Ibrahim H.⁹^ORCID

Affiliation:

1. Pharmacognosy and Medicinal Plants Department, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo 11884, Egypt

2. Biopharmaceutical Products Research Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications (SRTA-City), Alexandria, Egypt

3. Analytical Chemistry, Department of Pharmaceutical Analytical Chemistry Faculty of Pharmacy, Al-Azhar University, Nasr City, Cairo, Egypt

4. Chemistry Department, Faculty of Science (boys), Al-Azhar University, Cairo 11884, Egypt

5. Zoology Department, Faculty of Science (Boys), Al-Azhar University, Cairo 11884, Egypt

6. Polymeric Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-CITY), New Borg El-Arab City 21934, Alexandria, Egypt

7. Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia

8. Department of Pharmaceutical Sciences, College of Pharmacy, AlMaarefa University, Riyadh 13713, Saudi Arabia

9. Pharmaceutical Medicinal Chemistry & Drug Design Department, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo 11884, Egypt

Abstract

Objective. This study aimed to investigate the preparation of patuletin-encapsulated chitosan nanoparticles (PT-CS-NPs) using the ionic gelation method, evaluate their potential as anticancer agents, and invent a new method of differential pulse voltammetric analysis for patuletin (PT). Methods. Computational studies were conducted to assess the affinity of PT for chitosan, confirming a promising interaction. PT-CS-NPs were synthesized based on the computational outputs, resulting in nanoparticles with an angular structure and an average size of 6.168 nm. The cytotoxicity of PT and PT-CS-NP was evaluated in breast and colon cancer cell lines, and selectivity indices were calculated to assess safety profiles. The electrochemical behavior of PT was also investigated using the Britton–Robinson buffer. Results. PT-CS-NP exhibited both potent cytotoxicity and a favorable safety profile, showing the most active and safest pattern of cytotoxicity among the tested compounds. The electrochemical oxidation of PT was observed at 0.531 V vs. Ag/AgCl at pH 4.0.0. The concentration of PT showed a linear relationship with the corresponding peak current over the range of

{(10.0 x 10}^{- 9} : {10.0 x 10}^{- 5} M)

M, with a minimum limit of detection of 3.4 × 10(−9)M. The proposed method successfully measured PT, with a relative standard deviation below 2%. Conclusion. The preparation of PT-CS-NP via the ionic gelation method resulted in angular nanoparticles with a promising anticancer activity and safety profiles. The electrochemical behavior of PT was characterized, and a reliable method for PT quantification was established.