Cytotoxicity and Genotoxicity Evaluation of Organochalcogens in Human Leucocytes: A Comparative Study between Ebselen, Diphenyl Diselenide, and Diphenyl Ditelluride

Abstract

Organochalcogens, particularly ebselen, have been used in experimental and clinical trials with borderline efficacy. (PhSe)2and (PhTe)2are the simplest of the diaryl dichalcogenides and share with ebselen pharmacological properties. In view of the concerns with the use of mammals in studies and the great number of new organochalcogens with potential pharmacological properties that have been synthesized, it becomes important to develop screening protocols to select compounds that are worth to be testedin vivo. This study investigated the possible use of isolated human white cells as a preliminary model to test organochalcogen toxicity. Human leucocytes were exposed to 5–50 μM of ebselen, (PhSe)2, or (PhTe)2. All compounds were cytotoxic (Trypan’s Blue exclusion) at the highest concentration tested, and Ebselen was the most toxic. Ebselen and (PhSe)2were genotoxic (Comet Assay) only at 50 μM, and (PhTe)2at 5–50 μM. Here, the acute cytotoxicity did not correspond within vivotoxicity of the compounds. But the genotoxicity was in the same order of thein vivotoxicity to mice. These results indicate thatin vitrogenotoxicity in white blood cells should be considered as an early step in the investigation of potential toxicity of organochalcogens.