Affiliation:
1. Department of Parasitology, School of Basic Medical Sciences, Zunyi Medical University, Zunyi, China
Abstract
Cysticercosis is a cosmopolitan zoonotic parasitic disease infected by larval ofTaenia solium(T. solium). Several drugs for the treatment of cysticercosis, such as praziquantel, albendazole, and mebendazole, have certain toxicity and side effects. Considering that there is no vaccine available, we studied a new vaccine for cysticercosis in this study. The completeTSOL18gene and the optimizedSP-TSOL18gene fragments were obtained using PCR-based accurate synthesis method. The secretory and intracellular recombinant pMG36e-SP-TSOL18/Lactococcus lactis(L. lactis) and pMG36e-TSOL18/L. lactisvaccines ofT. soliumwere prepared. Immune responses in mice orally immunized with these two recombinantL. lactisvaccines were analyzed by the determination of specific antibodies (IgG, IgG1, IgG2a, and sIgA) in serum, spleen lymphocyte proliferation, and cytokines (IL-2, IFN-γ, IL-4, and IL-10) in spleen lymphocyte culture supernatant. Our results showed that, after the first immunization, in these two recombinantL. lactisvaccine groups, the levels of serum specific IgG, IgG2a, and IgG1 increased on 14–56 d and reached the highest level on days 42, 42, and 28, respectively. The level of specific sIgA of intestinal mucosa also increased on 14–56 d and reached the highest level on day 42. The level of spleen lymphocyte proliferation increased on 14–56 d and reached the highest level on day 42. The levels of IL-2, IFN-γ, IL-4, and IL-10 in spleen lymphocyte culture supernatant increased on 14–56 d and reached the highest level on days 42, 42, 28, and 28, respectively. These results indicated that the recombinant pMG36e-SP-TSOL18/L. lactisand pMG36e-TSOL18/L. lactisvaccines can induce specific cellular, humoral, and mucosal immune responses in mice with oral vaccination. More importantly, the recombinant pMG36e-SP-TSOL18/L. lactisvaccine has a better immune effect. In summary, these results demonstrated the possibility of usingL. lactisstrain as a vector to deliver protective antigens ofT. solium.
Funder
Science and Technology Cooperation Special Fund Project of Province and City
Subject
Immunology,General Medicine,Immunology and Allergy
Cited by
9 articles.
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