Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells-Reference-Cited by-同舟云学术

Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells

Published:2013 Issue: Volume:2013 Page:1-8
ISSN:2314-6133
Container-title:BioMed Research International
language:en
Short-container-title:BioMed Research International

Author:

Gao Yuhua¹²,Bai Chunyu¹²,Xiong Hui¹,Li Qian¹²,Shan Zhiqiang¹,Huang Linsheng¹,Ma Yuehui¹,Guan Weijun¹

Affiliation:

1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

2. College of Wildlife Resources, Northeast Forestry University, Harbin 150040, China

Abstract

Dermis-derived mesenchymal stem/progenitor cells (DMS/PCs) were isolated from the skin tissue of 16-day-old chick embryos and then characterized by immunofluorescence and RT-PCR. We found that primary DMS/PCs could be expanded for 15 passages. Expression ofβ-integrin, CD44, CD71, and CD73 was observed by immunofluorescence and RT-PCR. Passage 3 DMS/PCs were successfully induced to differentiate into osteoblasts, adipocytes, and neurocytes. The results indicate the potential for multilineage differentiation of DMS/PCs that may represent an ideal candidate for cellular transplantation therapy.

Funder

Ministry of Agriculture of China for Transgenic Research Program

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

http://downloads.hindawi.com/journals/bmri/2013/626258.pdf

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