Trueperella pyogenesandBrucella abortusCoinfection in a Dog and a Cat on a Dairy Farm in Egypt with Recurrent Cases of Mastitis and Abortion

Author:

Wareth Gamal12ORCID,El-Diasty Mohamed3,Melzer Falk1,Murugaiyan Jayaseelan4,Abdulmawjood Amir5ORCID,Sprague Lisa D.1,Neubauer Heinrich1

Affiliation:

1. Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany

2. Department of Pathology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Egypt

3. Animal Health Research Institute-Mansoura Provincial Laboratory, Mansoura, Egypt

4. Institute of Animal Hygiene and Environmental Health, Centre for Infectious Medicine, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13, 14163 Berlin, Germany

5. Institute of Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, Germany

Abstract

Trueperella pyogeneswas isolated from a dog and a cat with a mixed infection withBrucella abortus. Both lived on a dairy cattle farm with a history of regular cases of abortion and mastitis. Identification of the bacteria was done by means of MALDI-TOF MS, loop-mediated isothermal amplification (LAMP) based oncpn60, partial 16S rRNA sequencing, and growth on Loeffler Serum Medium. Isolation ofTrueperella pyogeneson the dairy farm highlights its neglected role in reproduction failure and draws attention to its effects in the dairy industry in Egypt. Diagnosis and control of abortion in Egypt should includeTrueperella pyogenesas one of possible causes of abortion.

Funder

Academy of Scientific Research and Technology

Publisher

Hindawi Limited

Subject

General Veterinary

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